BIOL 4340 Written Assignment 4 on Protein Trafficking **** SUBMIT ON D2L******
BIOL 4340 Written Assignment 4 on Protein Trafficking
**** SUBMIT ON D2L******
DUE Friday 3/29 [Good Friday, no classes]
Provide enough detail to complete 2 – 3 pages double spaced, which includes the figure that you find and discuss from current literature. Cite your resources.
Part 1:
Having made it through Cell Biology thus far, you now know enough about cells and their molecular mechanisms. There are approximately 10,000 proteins in a typical cell that are synthesized through different routes of intracellular protein trafficking. Each of you will be given a different protein and its final location in the cell.
Student (B ID)
Protein
Location
2337034
Uric Acid oxidase
Peroxisome
2417607
Na+/K+ pump
Outer cell membrane (Transmembrane protein)
2527650
S2 subunit of ribosomal protein
Assembled in nucleolus
2528223
Protein kinase C (PKC)
Cell membrane
2555892
Acid lipase
Lysosome
2568253
B-hexoaminidase
Lysosome
2582995
collagen
Found extracellularly
2587909
TOC complex (translocon)
Outer chloroplast membrane
2596579
L10a ribosomal protein
Found in cytosol as part of ribsosome
2599607
Potassium channel
Outer cell membrane (Transmembrane protein)
2615172
O-GlcNAc transferase
Golgi
2617058
RPOA alpha subunit in RNA polymerase
Nucleus
2645370
Glucosidase
Endoplasmic reticulum membrane
2645658
Cadherin
Found on outside cell membrane
2652975
Transferrin
Extracellular
2656082
TFII B protein
Found in nucleus
2657153
CD8 protein
Outer cell membrane
2660300
Fumerase
Mitochondria (Matrix)
2666106
Nuclear pore protein
Nucleus outer membrane
2667190
Rhodopsin
Outer Cell membrane
2667514
Catalase
Peroxisome
2667563
Succinyl CoA synthetase
Mitochondria (matrix)
2667657
RuBisCO
Chloroplast stroma
2667839
Succinate dehydrogenase
Mitochondria (inner membrane
2668018
Mannosidase
Endoplasmic reticulum membrane
2669625
Monoamine oxidase
Mitochondrial outer membrane
2705924
Signal peptidase
Endoplasmic reticulum
2712077
Xanthine oxidase
Cytosol
2712349
Acetyl CoA oxidase
Peroxisome
2733165
Adenylate cyclase
Outer cell membrane (cytosolic)
2738054
Galactose transferase
Golgi membrane
2742051
ATP synthase
Mitochondria (inner membrane)
2742172
Citrate synthase
Mitochondria matrix
2744256
Acetoacetyl-CoA synthase
Cytosol
2744828
Arp2/3 complex
Cytoskeleton
2744979
Aquaporin
Outer cell membrane (Transmembrane protein)
Part A:
Starting from the mRNA that is produced and released into the cytosol, describe in detail:
how your protein is translated and where this occurs.
how your protein is being processed and where
how it is being transported or further processed until it reaches its final destination.
Depending on your protein, many will have very different routes that they follow. This can be done by focusing in detail the more complex steps;
What mode(s) of protein trafficking occurs (translocation, gated transport or vesicular transport)?
Is your protein glycosylated (and where does that happen)?
Does it have disulfide bonds?
If it goes to the nucleus, what size is the protein?
If it simply stays in cytosol, then you will need to provide more detail about its transcription and translation than given in class (like about where each of the intermediate steps, like post transcriptional or post translational modifications, occur).
If it goes to several organelles, just focus detail on one part of the process.
If you are having trouble with this assignment, contact me. However, do your best at trying on your own first (for at least like an hour) so a meaningful discussion can occur.
Part B:
Look up a figure from primary literature (look under Pubmed) related to your protein. This figure should not be a summary diagram such as ones found in textbooks. Instead, it should be data (graphs, charts, diagrams, etc) relating to or including the protein itself (not the gene, either). If the figure is really large, you can just focus on 1-2 panels. Interpret the figure and its results.
For example, in the figure below; Int. J. Mol. Sci. 2019, 20, 5039. This represents a western blot to quantify the amount of Glucose-6-Phophatase (G6P) in the intestine as compared to the liver. The Ponceau at the bottom shows the same amount of protein was present in each lane meaning equal loading. The western blot itself is shown in B (Above it is a graphical representation of three determinations). The graph to the left and three lanes below are from the liver. The graph to the right and remaining lanes below are from the intestine. Of the three species, human intestine has the highest levels of G6P.
.